miRNA polymorphisms and risk of premature coronary artery disease
Agiannitopoulos, Konstantinos
Samara, Pinelopi
Papadopoulou,
Miranta
Efthymiadou, Astradeni
Papadopoulou, Eirini and
Tsaousis, Georgios N.
Mertzanos, George
Babalis, Dimitrios and
Lamnissou, Klea
Objective: Several microRNA (miRNA) polymorphisms have been associated
with susceptibility to specific health disorders, including
cardiovascular diseases. The aim of the present study was to investigate
whether four well-studied miRNA polymorphisms in non-Caucasian
populations, namely miR146a G>C (rs2910164), miR149 C>T (rs2292832),
miR196a2 C>T (rs11614913) and miR499 A>G (rs3746444), contribute to the
risk for the development of premature Coronary Artery Disease (CAD) in
the Greek population.
Methods: We used a case-control study to examine these associations in
400 individuals: 200 CAD patients [including a subgroup of myocardial
infraction (MI) patients] and 200 healthy controls, all of Greek origin.
MiRNA polymorphisms were genotyped using three different assays:
Polymerase chain reaction restriction fragment length polymorphism
(PCR-RFLP), High resolution Melting (HRM) and Sanger sequencing.
Results: Two of these polymorphisms, miR196a2 C>T (rs11614913) and
miR499 A>G (rs3746444) were found to be strongly associated with
increased risk for CAD (p=0.0388 and p=0.0013, respectively) and for MI
(p=0.0281 and p=0.0273, respectively). Furthermore,
miR146C-miR149C-miR196T-miR499G allele combination appeared to be
significantly related to CAD (p=0.0185) and MI (p=0.0337) prevalence.
Conclusions: Our results suggest that at least two of the studied
polymorphisms, miR196a2 C>T (rs11614913) and miR499 A>G (rs3746444), as
well as the miR146C-miR149C-miR196T-miR499G allele combination could
represent useful biomarkers of CAD and/or MI susceptibility in the Greek
population. These special genetic characteristics, in combination with
environmental factors and personal habits, might contribute to CAD
and/or MI prevalence. (C) 2020 Hellenic Society of Cardiology.
Publishing services by Elsevier B.V.
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