miRNA polymorphisms and risk of premature coronary artery disease

miRNA polymorphisms and risk of premature coronary artery disease

Agiannitopoulos, Konstantinos Samara, Pinelopi Papadopoulou, Miranta Efthymiadou, Astradeni Papadopoulou, Eirini and Tsaousis, Georgios N. Mertzanos, George Babalis, Dimitrios and Lamnissou, Klea

Objective: Several microRNA (miRNA) polymorphisms have been associated with susceptibility to specific health disorders, including cardiovascular diseases. The aim of the present study was to investigate whether four well-studied miRNA polymorphisms in non-Caucasian populations, namely miR146a G>C (rs2910164), miR149 C>T (rs2292832), miR196a2 C>T (rs11614913) and miR499 A>G (rs3746444), contribute to the risk for the development of premature Coronary Artery Disease (CAD) in the Greek population. Methods: We used a case-control study to examine these associations in 400 individuals: 200 CAD patients [including a subgroup of myocardial infraction (MI) patients] and 200 healthy controls, all of Greek origin. MiRNA polymorphisms were genotyped using three different assays: Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), High resolution Melting (HRM) and Sanger sequencing. Results: Two of these polymorphisms, miR196a2 C>T (rs11614913) and miR499 A>G (rs3746444) were found to be strongly associated with increased risk for CAD (p=0.0388 and p=0.0013, respectively) and for MI (p=0.0281 and p=0.0273, respectively). Furthermore, miR146C-miR149C-miR196T-miR499G allele combination appeared to be significantly related to CAD (p=0.0185) and MI (p=0.0337) prevalence. Conclusions: Our results suggest that at least two of the studied polymorphisms, miR196a2 C>T (rs11614913) and miR499 A>G (rs3746444), as well as the miR146C-miR149C-miR196T-miR499G allele combination could represent useful biomarkers of CAD and/or MI susceptibility in the Greek population. These special genetic characteristics, in combination with environmental factors and personal habits, might contribute to CAD and/or MI prevalence. (C) 2020 Hellenic Society of Cardiology. Publishing services by Elsevier B.V. (EN)

scientific_publication_article

Επιστημονική δημοσίευση - Άρθρο Περιοδικού (EL)

Scientific publication - Journal Article (EN)

University of Athens

Pergamos Digital Library

Journal Article

English

2021-01-01

uoadl:3029652

https://pergamos.lib.uoa.gr/uoa/dl/object/uoadl:3029652