Micro-injection of morpholino oligonucleotides for depleting securin in mouse oocytes

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Micro-injection of morpholino oligonucleotides for depleting securin in mouse oocytes (EN)

Marangos, P. (EN)

Πανεπιστήμιο Ιωαννίνων. Σχολή Θετικών Επιστημών. Τμήμα Μηχανικών Η/Υ & Πληροφορικής (EL)
Marangos, P. (EN)

Gene silencing techniques have brought new insights into mammalian oocyte and embryo development. More specifically, the use of Morpholino oligonucleotides which sterically inhibit translation from target mRNAs thereby compromising gene function, allowed the identification of important oocyte regulators and especially factors involved in meiotic cell cycle control. Here we describe the method of application of Morpholino oligonucleotides in mouse oocyte research (EN)

bookChapter

Morpholino (EN)


English

2013

http://olympias.lib.uoi.gr/jspui/handle/123456789/26613

Humana Press (EN)





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